華中農(nóng)業(yè)大學性命科技進步學校戰(zhàn)士娟博士研究生為第一作者、王革嬌專家教授與史凱祥副研究員為一同通訊作者，該工作中獲得自然科學基金的支助。

　　英文摘要：

　　In this study, we isolated a highly cadmium （Cd）-resistant bacterium, Pseudomonassp. B7, which immobilized 100% Cd（II） from medium. Culturing strain B7 with Cd（II） led to the change of functional groups, mediating extracellular Cd（II） adsorption. Proteomics showed that a carbonic anhydrase, CadW, was upregulated with Cd（II）。 CadW expression in Escherichia coli conferred resistance to Cd（II） and increased intracellular Cd（II） accumulation. Fluorescence assays demonstrated that CadW binds Cd（II） and the His123 residue affected Cd（II） binding activity, indicating that CadW participates in intracellular Cd（II） sequestration. Chinese cabbage pot experiments were performed using strain B7 and silicate [Si（IV）]. Compared with the control, Cd content in aboveground parts significantly decreased by 21.3%, 29.4% and 32.9%, and nonbioavailable Cd in soil significantly increased by 129.4%, 45.0% and 148.7% in B7, Si（IV） and B7 Si（IV） treatments, respectively. The application of Si（IV） alone reduced chlorophyll content by 20.8% and arylsulfatase activity in soil by 33.9%, and increased malonaldehyde activity by 15.0%. The application of strain B7 alleviated the negative effect of Si（IV） on plant and soil enzymes. Overall, application of Si（IV） is most conducive to the decreased Cd accumulation in plant, and strain B7 is beneficial to maintaining soil and plant health.

　　原文連接：https://www.sciencedirect.com/science/article/pii/S0304389421028880